Inorganic pyrophosphatase induces type I collagen in osteoblasts
نویسندگان
چکیده
منابع مشابه
Yeast Inorganic Pyrophosphatase
The kinetics of the Mg2+ activation of crystalline yeast inorganic pyrophosphatase have been thoroughly investigated from pH 7.40 to pH 9.05 using a sensitive isotope assay in order to determine the role of the divalent metal ion activator in the reaction. A computer program has been devised for calculating the concentration of each of the various components of the complex equilibrium involving...
متن کاملYeast Inorganic Pyrophosphatase
The magnesium ion-dependent hydrolysis of pyrophosphate catalyzed by yeast inorganic pyrophosphatase is markedly inhibited by calcium ion. The kinetics of inhibition have been measured and analyzed in terms of a model involving strong interactions between CaPPi and two metalcomplexed forms of the enzyme, EMg and ECa. Inhibition constants for these interactions have been estimated by nonlinear r...
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Bone and dentin are mineralized extracellular matrices produced by osteoblasts and odontoblasts, respectively, and their major organic portion is type I collagen. Dentinogenesis Imperfecta (DGI) is one of the most common clinically- and genetically-based disturbances of dentin formation, causing irreversible dentin defects. Among several types of DGI, patients with DGI type II exhibit opalescen...
متن کاملPurification of yeast inorganic pyrophosphatase.
An enzyme which hydrolyzes inorganic pyrophosphate (PP) to orthophosphate (ortho P) was demonstrated by Lohmann (1) in muscle. Bauer (2) found that autolysates of slowly dried bottom yeast were a rich source of inorganic pyrophosphatase and were free of adenosinetriphosphatase (ATPase) activity. An important contribution was made by Bailey and Webb (3) who accomplished an extensive purification...
متن کاملConstitutive Inorganic Pyrophosphatase of Escherichia coli
An intracellular, inorganic pyrophosphatase from Escherichia co2i K-12 3000(X) has been purified 500-fold to a state of apparent homogeneity. The enzyme catalyzes hydrolysis of inorganic pyrophosphate, tripolyphosphate, and tetrapolyphosphate with relative velocities of 1.000, 0.016, and 0.007, respectively. No activity whatsoever was found with a variety of other phosphate esters. There was an...
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ژورنال
عنوان ژورنال: Bone
سال: 2010
ISSN: 8756-3282
DOI: 10.1016/j.bone.2009.08.055